There is a big difference between PBS and HBSS. While both options have their pros and cons, it’s important to know what those are before you make your decision. In this post, we’ll outline the main differences between PBS and HBSS so you can make the best choice for your business.
What is PBS?
PBS, or phosphate-buffered saline, is a salt solution that is commonly used in biomedical research. PBS is buffered with a phosphate buffer to maintain a constant pH, and it also contains saline (salt) to keep cells hydrated. PBS is often used to wash cells or tissue before experiments, and it can also be used to dilute cells or proteins for storage. PBS is non-toxic and relatively inexpensive, making it a popular choice for researchers. PBS can also be used as a control in many experiments, as its composition is well-characterized and consistent across different batches. PBS is an important tool in biomedical research, and it has many uses in the laboratory.
What is HBSS?
HBSS Hank’s balanced salt solution is a saline solution that is used to maintain the osmotic balance in cells. The solution is also used to wash away excess or unwanted material from surfaces. HBSS Hank’s balanced salt solution is isotonic, which means that it has the same osmotic pressure as blood plasma. This makes it an ideal solution for maintaining cell integrity and for reducing the risk of cell lysis. HBSS Hank’s balanced salt solution is available in a variety of formulations, and it can be customized to meet the specific needs of cell culture.
Difference between PBS and HBSS
PBS and HBSS both refer to buffered saline solutions that are commonly used in biological research. PBS stands for phosphate-buffered saline, while HBSS stands for Hank’s balanced salt solution. PBS is typically used for tissues that are sensitive to changes in pH, while HBSS is generally used for cells that require a more accurate osmotic balance. PBS solutions contain NaCl, KH2PO4, and Na2HPO4. HBSS solutions contain NaCl, KCl, CaCl2, MgCl2, and dextrose. PBS solutions have a pH of 7.4, while HBSS solutions have a pH of 7.2.
Conclusion
Hank’s balanced salt solution, or HBSS, is more physiological saline than PBS because it contains all of the ions found in human blood. This means that it can be used to study how cells respond to different environments and stimuli. For these reasons, HBSS is often used in place of PBS for many cellular experiments.
